Laboratories of Molecular Medicine (LMM)
The Page laboratory is housed in a new 105,000 sq. ft. research laboratory, the Laboratories of Molecular Medicine, LMM, with more than 750 sq. feet of individual wet laboratory space plus an additional room for computation work and a separate data collection facility. The laboratories in LMM are open-lab-space facilities, allowing close interaction with the adjunct structural biology and molecular biology groups at Brown University. LMM also houses a number of Core Facilities, including the newly established Structural Biology Core Facility (SBCF), the Proteomics and Biophysical Characterization Facility and the Genomics Core Facility. Shared biophysical equipment includes a Microcal iTC200, Microcal VP-DSC, Jasco J-815 CD, Fluor-Max, LTQ Oribitrap Velox and AKTAprime Plus.
State-of-the-Art Crystallography InstrumentationThe newly established Structural Biology Core Facility (SBCF) houses the new X-ray crystallography, small angle X-ray scattering and NMR instrumentation. It is directed by Dr. Michael Clarkson, Assistant Professor Research, who has more than 15 years of experience in NMR spectroscopy, SAXS and crystallography techniques. In July 2012, a Rigaku 'Ultimate Home Lab' system was installed, which includes an FRE+ Superbright microfocus generator coupled with the Varimax-HF Arc)Sec confocal optics, with a Saturn 944HG CCD detector and an ACTOR crystal mounting robot on one port for crystal diffraction and a BioSAXS-100 SD-Kratky Camera System on the second port for solution scattering. Additional crystallography equipment in the laboratory includes an Art Robbins Phenix nanoliter crystallization robot, a CrysCam imaging system and both ALS and Uni Pucks for automated crystal screening and data collection both at Brown and at Synchrotrons. The SBCF also houses the NMRs, which includes a Bruker AVANCEII 500 MHz spectrometer with a TCI HCN z-gradient cryoprobe and, as of 11/2012, a Bruker AVANCEII 850 MHz spectrometer.
New NMR Instrumentation to arrive 11/2012
The SBCF also houses the NMR spectrometers, which includes a Bruker AVANCEII 500 MHz spectrometer with a TCI HCN z-gradient cryoprobe and, as of 11/2012, a Bruker AVANCEII 850 MHz spectrometer which will also have a TCI HCN z-gradient cryoprobe .
LMM | Space and Equipment
LMM | Page Laboratory
The laboratory has ample space and is equipped with modern protein production and purification instrumentation for sample production. Instrumentation includes -20/-80 freezers, electrophoresis systems (DNA/protein), Eppendorf Mastercycler PCR gene amplification systems, multiple refrigerated incubator shakers for large-scale expression (up to 50 L per day), Vertiga Micro-expression Device, GE Healthcare Akta Purifiers (3) and Akta Primes (2), Beckman X-15 centrifuge, Avestin EmulsiFlex-C3 Cell Cracker, a MicroCal VP-200 and a Biotek EPOCH UV plate reader, among others. Individual laboratory benches are available for all laboratory members. Windows-based PCs are available for data analysis, graphic manipulation and report preparation. Two LINUX workstations, housed in a dedicated model-building room, are available for X-ray crstyallography data processing, building and refinement (includes commonly used programs, such as HKL3000, Phenix, CCP4, Coot, Pymol, among others.